Coding
Tc2bh

Part:BBa_J212006

Designed by: Xing Yan   Group: Zhou Lab   (2012-02-03)


Exo2b catalytic domain fused with the cbh1 gene

A novel GH5 cellulase gene exo2b was identified from a metagenomic library. The fragment encoding the Exo2b catalytic domain was then fused with the cbh1 gene from Trichoderma reesei, and the fused gene was successfully expressed in T. reesei Rut-C30 .Compared to that of the parent strain, the filter paper activity (FPA) and CMCase activity of the secreted proteins of a selected transformant A1, increased 24% and 18%, respectively.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 406
    Illegal EcoRI site found at 1060
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 406
    Illegal EcoRI site found at 1060
    Illegal NheI site found at 2193
    Illegal NheI site found at 2320
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 406
    Illegal EcoRI site found at 1060
    Illegal BglII site found at 758
    Illegal BamHI site found at 1069
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 406
    Illegal EcoRI site found at 1060
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 406
    Illegal EcoRI site found at 1060
    Illegal NgoMIV site found at 1960
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 244
    Illegal BsaI.rc site found at 1204


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